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KMID : 0380619890210050606
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Korean Journal of Food Science and Technology
1989 Volume.21 No. 5 p.606 ~ p.612
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Purification and Biophysical Characterization of New Lectin from Baby Clam , Tapes japonica
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Abstract
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A New lectin from baby clam, Tapes japonica, was isolated and purified through the following procedures; acetone powder, 0.15M NaCI extraction, ammonium sulfate fractionation, N-acetylD-galactosamine-agarose affinity column, and ion exchange Mono Q of FPLC. This lectin nonspecifically agglutinated human erythrocytes but didn´t agglutinate mouse and rabbit erythrocytes. And the lectin neither stimulated human lymphocytes nor agglutinated Sarcoma 180 cells. On polyacrylamide gel electrophoresis, the lectin migrated as a major single band indicating homogeneous. A molecular weight was estimated to be about 131,000 daltons by Biogel P-300 and 125,000 daltons by SDS-PAGE without β-mercaptoethanol. This lectin is supposed to be a tetramer composed of heterogeneous subunits, about 30,000 and 33,000 daltons. Baby clam lectin was inhibited by EDTA and recovered agglutinating activity by Ca^(++) and Mn^(++). This lectin is revealed as glycoprotein that contained about 4.2% neutral sugar.
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